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. 2019 Feb 28;9:3037. doi: 10.1038/s41598-019-39403-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amino acid- and position-specific redistribution of ribosomal density upon depletion of eEF3 or eIF5A. (a) Distribution of genomic sites (codons) according to the relative fold difference (FD) in the ribosome density between eEF3-deficient and eEF3-proficient cells. Positive FD values (right side of the distribution) indicate the sites where depletion of the factor leads to an increase in ribosome density, and negative FD values correspond to a relative decrease in ribosome density upon depletion of the factor. pLogo³⁹ was used to calculate overrepresentation of specific amino acids at positions relative to the P-site codon using codons with Z-score <-2 (eEF3: panel b, 651 codon positions; eIF5A³²: panel d, 510 codon positions) and >2 (eEF3: panel c, 856 codon positions; eIF5A³²: panel e, 998 codon positions) that are common for both biological replicates. Horizontal red lines on the pLogos represent significance threshold (the log₁₀-odds 3.45) corresponding to a Bonferroni corrected p-value of 0.05.