Fig. 4.

Hypoxia induces CREB-mediated EC proliferation and migration. (A) and (B) HPAEC proliferation following transfection with vehicle-scrambled or CREB siRNA under 24 h normoxia or hypoxia treatment was assayed by crystal violet absorbance, (n = 3–11) * **p < 0.001; and by cell cycle flow cytometry analysis comparing numbers of cells in S + G2-M phases across groups, (n = 4–6) *p < 0.05, respectively. (C) HPAEC migration following transfection with scrambled control or CREB siRNA under 24 h hypoxia vs. normoxia was assayed by wound injury (scratch assay) (n = 3); * *p < 0.01. Vertical black lines delineate cell fronts and residual wound sizes. Two-way ANOVA followed by Sidaks post-hoc test was performed.