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. 2019 Feb 14;22:101117. doi: 10.1016/j.redox.2019.101117

Fig. 7.

Fig. 7

EPI attenuated MCT-induced liver inflammatory injury in both wild-type and Nrf2 knock-out mice. Wild-type or Nrf2 knock-out mice were given with MCT (360 mg/kg) and EPI (40 mg/kg), and sacrificed at 48 h after MCT administration. (A) Liver MPO activity (n = 6). (B) Hepatic expression of IκB, p-IκB and NFκB. (C) The quantitative densitometric analysis of nuclear NFκB (n = 5). (D) The quantitative densitometric analysis of IκB (n = 5). (E) The quantitative densitometric analysis of p-IκB (n = 5). (F) TNFα, IL-6 and IL-1β mRNA expression (n = 5). (G) Liver immunohistochemical staining of Gr-1. (Arrows indicate Gr-1-positive cells). Left figure is the number of Gr-1-positive neutrophils counted manually (n = 3). (H) Liver immunohistochemical staining of CD11b. (Arrows indicate CD11b-positive cells). Left figure is the number of CD11b-positive macrophages counted manually (n = 3). Data are expressed as mean ± SEM. *P < 0.05 versus control; #P < 0.05 versus MCT; &P < 0.05 versus wild-type mice.