FIG 2.
Phylogenetic relationships among the CPE isolates with IncFII-type plasmids harboring blaNDM. All of the isolates, except for two blaNDM-4 carriers (†), harbored the blaNDM-5 gene on the IncFII-type plasmid. Environmental isolates are marked in cyan. blaNDM-harboring plasmids were detected by S1-PFGE-Southern blot analysis with a blaNDM probe. Red rectangles indicate closely related isolates. Cluster A, 7 to 20 SNPs, >99.9% ANI; cluster B, 6 to 22 SNPs, >99.9% ANI; cluster C, 8 to 28 SNPs, >99.9% ANI. SNPs between the isolates in cluster B and the other ST167 isolates were 833 to 1,051. Genome sequences of E. coli K-12 MG1655 and K. pneumoniae AATZP (GenBank accession number CP014755.1) were used as references for construction of the phylogenetic trees of respective species. Isolate M214 carried an IncA/C2 plasmid harboring blaNDM-1 (176 kb) in addition to an IncFII plasmid harboring blaNDM-5 (95 kb). ‡, M520B and M520R were isolated from the same specimen. -, unknown.