TABLE 5.
Affinities of binding of cyclopeptides to full-length ClpC1 determined by SPRa
| Target strain | Inhibitor | ka (1/Ms) | kd (1/s) | KD (nM) |
|---|---|---|---|---|
| M. tuberculosis | RUFI | 2.98 × 106 ± 1.8% | 3.01 × 10−1 ± 1.7% | 101 ± 1.8 |
| ECU | 6.12 × 104 ± 0.7% | 6.14 × 10−3 ± 5.4% | 100 ± 4.8 | |
| CYMA | 4.36 × 106 ± 1.8% | 2.48 × 10−2 ± 1.5% | 5.69 ± 0.09 | |
| M. abscessus | RUFI | 1.42 × 106 ± 1.3% | 1.37 × 10−1 ± 5.6% | 96.4 ± 3.3 |
| ECU | 5.66 × 104 ± 4.7% | 7.01 × 10−3 ± 0.4% | 125 ± 3.2 | |
| CYMA | 5.68 × 106 ± 1.5% | 2.19 × 10−2 ± 1.3% | 3.85 ± 0.05 | |
| M. chelonae | RUFI | 2.96 × 105 ± 0.6% | 2.13 × 10−2 ± 0.4% | 72.1 ± 0.4 |
| ECU | 5.34 × 104 ± 2.9% | 6.72 × 10−3 ± 1.3% | 126 ± 2.7 | |
| CYMA | 7.60 × 106 ± 14% | 2.45 × 10−2 ± 13% | 3.23 ± 0.44 |
a
Percent standard deviations were calculated from two or three independent experiments.