FIG 11.

Compounds 7, 135, and 201 inhibit B19V replication in CD36⁺ EPCs. CD36⁺ EPCs were infected with B19V, followed by addition of each compound, compound 7 (A), compound 135 (B), and compound 201 (C), at various concentrations, as shown. At 2 days postinfection, Hirt DNA samples were prepared from infected cells and were subjected to Southern blotting using the M20 probe. DMSO was used as a vehicle control. CD36⁺ EPCs without infection were set up as mock-infected cells (Mock). pM20 digested with SalI was loaded as a size marker (Marker). Mitochondrial (mt) DNA was detected as a loading control using a specific mt DNA probe (91). *, monomer replicative-form DNA; **, ssDNA, single-stranded DNA.