FIG 3.

Compound screening for inhibition of cleavage of the ³²P-labeled B19V Ori30 oligonucleotide. A total of 96 small-molecule compounds at 100 µM (A) or the chosen 25 compounds at 10 µM (B) were incubated with ³²P-labeled Ori30 and NS1N in the nicking buffer. The reaction mixtures were analyzed on a denaturing polyacrylamide gel. The relative nicking efficiencies are shown beneath the gels. The ratio of the signals at 19 nt versus those at 19 nt plus 30 nt in the DMSO vehicle control group is set equal to 1. (A) The nicking efficiency for compounds that inhibited all nicking activity is shown as a blank, as no obvious nicked bands were detected at 19 nt. (B) Autoradiography gel images for the 25 compounds assessed at 10 µM are shown.