TABLE 3.
Micafungin and anidulafungin MICs for the isolates in each used pair to prepare the spiked suspensions in Bactec bottles and FKS2 gene sequence of the tested isolatesa
| Pair | Isolate | EUCAST MYC/AND MIC (mg/liter) | FKS2 gene sequence |
|---|---|---|---|
| 1 | Parental | 0.015/0.032 | WT |
| Isogenic | 4/2 | ΔF658 | |
| 2 | Parental | 0.015/0.032 | WT |
| Isogenic | 4/2 | ΔF658 | |
| 3 | Parental | 0.015/0.032 | WT |
| Isogenic | 4/2 | S663P | |
| 4 | Parental | 0.015/0.032 | WT |
| Isogenic | 0.25/0.5 | E655A | |
| 5 | Parental | 0.015/0.032 | WT |
| Isogenic | 0.064/0.25 | WT | |
| 6 | Parental | 0.015/0.015 | WT |
| Isogenic | 0.5/0.5 | W715L | |
| 7 | Parental | 0.015/0.015 | WT |
| Isogenic | 2/1 | ΔF658 | |
| 8 | Parental | 0.015/0.064 | WT |
| Isogenic | 1/2 | S663Y | |
| 9 | Parental | 0.015/0.032 | WT |
| Isogenic | 0.064/0.5 | D666N | |
| 10 | Parental | 0.015/0.032 | WT |
| Isogenic | 2/1 | S663P |
MYC, micafungin; AND, anidulafungin. Parental isolates were phenotypically echinocandin susceptible, and isogenic ones were phenotypically echinocandin resistant. Pairs 1 to 8 came from a previous study and involved susceptible isolates from blood samples exposed in vitro to either micafungin or anidulafungin and the corresponding resistant ones generated (27, 28). Pairs 9 and 10 originated in two patients with candidemia who developed concomitant echinocandin-resistant endocarditis. The parental and isogenic isolates in each pair proved to be genotypically identical. WT, wild type.