Table III.

Multiplex-PCR amplification primers of the IGH complementarity-determining region 3 region.

Primer name	Sequence (5′-3′)
IGHV1-18	CAGACGTGTGCTCTTCCGATCTAGAGAGTCACCATGACCACAGAC
IGHV1-2/1-46	CAGACGTGTGCTCTTCCGATCTAGAGAGTCACCAKKACCAGGGAC
IGHV1-24	CAGACGTGTGCTCTTCCGATCTAGAGAGTCACCATGACCGAGGAC
IGHV1-3/1–45	CAGACGTGTGCTCTTCCGATCTAGAGAGTCACCATTACYAGGGAC
IGHV1-69/1-f	CAGACGTGTGCTCTTCCGATCTAGAGAGTCACGATWACCRCGGAC
IGHV1-8	CAGACGTGTGCTCTTCCGATCTAGAGAGTCACCATGACCAGGAAC
IGH2-70/26/5	CAGACGTGTGCTCTTCCGATCTAGACCAGGCTCACCATYWCCAAGG
IGHV3	CAGACGTGTGCTCTTCCGATCTAGGGCCGATTCACCATCTCMAG
IGH4	CAGACGTGTGCTCTTCCGATCTAGCGAGTCACCATRTCMGTAGAC
IGHV5-51	CAGACGTGTGCTCTTCCGATCTAGCAGCCGACAAGTCCATCAGC
IGHV6-1	CAGACGTGTGCTCTTCCGATCTAGAGTCGAATAACCATCAACCCAG
IGHV7-NEW	CAGACGTGTGCTCTTCCGATCTAGGACGGTTTGTCTTCTCCTTG
IGHJ-Rev1	CTACACGACGCTCTTCCGATCTCTGAGGAGACRGTGACCAGGGTG
IGHJ-Rev2	CTACACGACGCTCTTCCGATCTCTGAAGAGACGGTGACCATTGTC
IGHJ-Rev3	CTACACGACGCTCTTCCGATCTCTGAGGAGACGGTGACCAGGGT
IGHJ-Rev4	CTACACGACGCTCTTCCGATCTTGAGGAGACGGTGACCGTGGTC

IGHV, immunoglobulin heavy chain variable; IGHJ, IGH joining; f, forward; Rev, reverse; Annexing primer bases: R=A or G, Y=C or T, K=G or T M=A or C, W=A or T.