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. 2019 Jan 21;17(3):2185–2193. doi: 10.3892/etm.2019.7191

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Copyright: © Lee et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Expression of adipogenesis and lipogenesis associated genes in liver tissue. HFD-induced obesity C57BL/6 mice were treated with Vehicle, OT (10 mg/kg) or EMfC (50 mg/kg) for 12 weeks. Reverse transcription-quantitative polymerase chain reaction using specific primers measured the expression level of genes associated with (A) adipogenesis and (B) lipogenesis in the liver. The intensity of each band was determined using an imaging densitometer, and the relative levels of each gene were calculated based on the intensity of actin. Data is presented as the mean ± standard deviation of the three experiments. *P<0.05 vs. the No treatment group; ^#P<0.05 vs. the HFD+Vehicle treated group. HFD, high fat diet; OT, Orlistat; EMfC, extract of mulberry leaves fermented with Cordyceps militaris; PPAR, peroxisome proliferator-activated receptor; C/EBP, CCAAT-enhancer binding protei; FAS, Fas cell surface death receptor; aP2, adipocyte protein 2.