Expression of lipolysis associated proteins in liver tissues. (A) Western blot analysis measured the phosphorylation or expression of several lipolysis associated proteins, including (B) perilipin, p-perilipin, HSL, p-HSL and ATGL. The intensity of each band was determined using an imaging densitometer, and the relative levels of the four proteins were calculated based on the intensity of actin. Five to six rats per group were assayed in triplicate by western blotting. Data are presented as the mean ± standard deviation of three replicates. *P<0.05 vs. the No treatment group; #P<0.05 vs. the HFD+Vehicle treated group. HFD, high fat diet; OT, Orlistat; EMfC, extract of mulberry leaves fermented with Cordyceps militaris; p-, phosphorylated; HSL, hormone-sensitive lipase; ATGL, adipose triglyceride lipase.