Figure 3.
miR-212 and HIF-1α mRNA expression levels are upregulated in in vitro under hypoxic conditions. (A) miR-212 and HIF-1α mRNA expression levels were measured using RT-qPCR following hypoxic stimulation in MiaPaca2 cells. (B) miR-212 and HIF-1α mRNA expression levels were measured using RT-qPCR following hypoxic stimulation in AsPc1 cells. HIF-1α protein expression levels were measured by western blotting following hypoxic stimulation in (C) MiaPaca2 and (D) AsPc1 cell lines. *P<0.05. miR, microRNA; HIF-1α, hypoxia-inducible factor-1α; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.