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. 2019 Feb 23;22:101152. doi: 10.1016/j.redox.2019.101152

Fig. 2.

Fig. 2

Isolation of mitochondria in hypoxia prevents oxidation of the glutathione pool. (A) Schematic drawing of the experiments performed in B-E. (B, C) Mitochondria from Grx1-roGFP2 cardiomyocyte specific transgenic hearts were isolated, incubated and analyzed in a defined oxygen environment as indicated. 11, 6, 6, and 11 hearts were included in the analysis at 20.9, 10, 3, and 0.1% O2, respectively. Subsequently the mitochondrial Oxidation Difference (mtOxD) and the glutathione redox potential (EGSH) were determined. The defined oxygen environment was continued during the mtOxD and EGSH measurements for each sample. (D, E) Mitochondria from Grx1-roGFP2 cardiomyocyte specific transgenic hearts were isolated either at 20.9% O2 or 0.1% O2. Subsequently, the mitochondria were incubated and analyzed at the same oxygen concentration (20.9% O2, 11 hearts), reoxygenated (0.1 → 20.9% O2, 6 hearts) or exposed to hypoxia (20.9 → 0.1% O2, 4 hearts) as indicated. *p < 0.05.