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. 2019 Feb 23;22:101152. doi: 10.1016/j.redox.2019.101152

Fig. 3.

Fig. 3

Addition of succinate/ADP results in a reduction of the glutathione pool in mitochondria isolated in hypoxia. Grx1-roGFP2 tg mitochondria were isolated in 20.9% O2 (4 hearts) or 0.1% O2 (4 hearts). One portion of the 0.1% O2 isolated mitochondria were incubated for 30 min at 20.9% O2 before addition of substrates (0.1%→20.9% 02). Subsequently, succinate and ADP were added for 30 min as indicated and the mitochondrial Oxidation difference (mtOxD, A) and EGSH (B) were determined. During substrate addition and mtOxD analysis the indicated oxygen concentration was continued for each sample. *p < 0.05.