Figure 2.

Effect of resveratrol on apoptosis in the human MES-SA uterus sarcoma cell line. Cells were treated with resveratrol for 72 h, and apoptosis was determined by flow cytometry using an Annexin V kit. (A) Quantified amounts of cells in early and late apoptosis in groups treated with different concentrations of resveratrol. (B-E) Representative flow cytometry dot plots of MES-SA cells treated with resveratrol at (B) 0 µg/ml, (C) 10 µg/ml, (D) 20 µg/ml and (E) 40 µg/ml. Quadrants: UL, necrotic cells; UR, late apoptotic cells; LL, live cells; LR, early apoptotic cells. PI, propidium iodide; UL, upper left; UR, upper right; LL, lower left; LR, lower right.