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. 2019 Jan 28;17(3):2221–2229. doi: 10.3892/etm.2019.7206

Figure 6.

Figure 6.

Effect of miR-150-5p inhibition on epithelial-mesenchymal transition in breast cancer cells. (A) The relative protein expression level of N-cadherin, vimentin, β-catenin, E-cadherin and ZO-1 were determined by western blot analysis in MDA-MB-468 cells following 48-h transfection with miR-150-5p inhibitor and inhibitor control. Quantification of (B) N-cadherin, (C) vimentin, (D) β-catenin, (E) E-cadherin and (F) ZO-1 protein expression. The relative (G) N-cadherin, (H) vimentin, (I) β-catenin, (J) E-cadherin and (K) ZO-1 mRNA expression level was determined by RT-qPCR in MDA-MB-468 cells following 48-h transfection with miR-150-5p inhibitor and inhibitor control. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01 vs. Control. miR, microRNA; ZO-1, tight junction protein ZO-1; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.