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. 2018 Dec 17;17(3):1509–1516. doi: 10.3892/etm.2018.7106

Figure 2.

Figure 2.

miR-375 suppresses cell proliferation and colony formation in KYSE450 and KYSE150 cells. (A) The mRNA expression levels of miR-375 in ESCC cell lines KYSE450 and KYSE150 were analyzed following transient transfection with miR-375 mimic, inhibitor and negative controls. The expression level of miR-375 was normalized to U6 small nuclear RNA. *P<0.05 and **P<0.01 as indicated. (B) The KYSE450, KYSE150, and Het-1A cell proliferation was analyzed by CCK-8 assay. *P<0.05 and **P<0.01 vs. Het-1A. (C) The colony formation in KYSE450, KYSE150, and Het-1A cell lines was analyzed (magnification, ×100). (D) The effect of miR-375 overexpression and knockdown on KYSE450 and KYSE150 cell proliferation was analyzed by CCK-8 assay. *P<0.05 and **P<0.01 vs. Het-1A. (E) The effect of miR-375 overexpression and knockdown on KYSE450 and KYSE150 colony formation was analyzed (magnification, ×100). miR, microRNA; ESCC, esophageal squamous cell carcinoma; OD, optical density; NC, negative control; CCK-8, cell counting kit; KYSE450 and KYSE150, ESCC cell lines; Het-1A, normal esophageal endothelial cell line; miR-375 mimic, ESCC cell (KYSE450 or KYSE150) transfected with miR-375 mimic; NC mimic, ESCC cell (KYSE450 or KYSE150) transfected with NC miRNA; miR-375 inhibitor, ESCC cell (KYSE450 or KYSE150) transfected with miR-375 inhibitor; NC inhibitor, ESCC cell (KYSE450 or KYSE150) transfected with NC siRNA.