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. 2019 Feb 22;12:47. doi: 10.3389/fnmol.2019.00047

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Copyright © 2019 Ilieva, Nielsen, Korshunova, Gotfryd, Bock, Pankratova and Michel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Artemin- and artefin-induced phosphorylation of RET receptor. Serum-starved PC12-E2 cells were stimulated with 2.1 nM ARTN or 12.64 μM artefin for 10 min. Non-treated cells served as control. Lysates were prepared and examined by immunoblot for phosphotyrosine and total RET after stripping of the membrane. The bar diagram shows the phosphorylation expressed as a ratio between phosphorylated tyrosine residues and amount of total RET. The results are shown as percentage of control set at 100% and presented as mean ± SEM. The asterisks indicate the significance levels: ^∗p < 0.05; ^∗∗p < 0.01.