FIGURE 7.

Neuritogenic effect of ARTN and artefin in (A) dominant negative RET or (B) knock down NCAM-expressing CGNs. CGNs were transfected with empty vector (ev), dominant negative version of RET (dnRet), or shNCAM-encoding plasmid (kdNCAM) and stimulated with P2-d (8 μg/ml), ARTN (0.21 nM), and artefin (1.4 μM) for 24 h. The results are presented as percentage of control set at 100%, and error bars indicate SEM. Student’s paired t-test was used for evaluation. + Presents p-values for comparison of the neuritogenic effect of compounds to the untreated neurons, while ^∗ shows p-values for comparison to the positive control of CGNs transfected with empty vector. ⁺p < 0.05; ⁺⁺p < 0.01; ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.