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. 2019 Feb 22;12:47. doi: 10.3389/fnmol.2019.00047

FIGURE 8.

FIGURE 8

Inhibition of ARTN- and artefin-induced neurite outgrowth in CGNs by FGFR-inhibitor SU5402. (A) Neurite outgrowth induced by P2-d peptide (8 μg/ml). + Presents p-values for comparison of the neuritogenic effect of the peptide to the untreated neurons, while ∗ shows p-values for comparison to the positive control of CGNs stimulated with P2-d. (B) Inhibition of neurite outgrowth with serially diluted SU5402 (20, 40, 80 μM) induced by ARTN (0.21 nM) (B) or artefin (1.4 μM) (C). ∗Shows p-values for comparison to the positive control of CGNs stimulated with ARTN or artefin. ++p < 0.01; ∗∗p < 0.01. (D) Inhibition of ARTN- and artefin-induced neuritogenic effect in dominant negative FGFR (dnFGFR) transfected CGNs. CGNs transfected with empty vector (ev) or dominant negative version of FGFR (dnFGFR) were plated and stimulated with P2-d (8 μg/ml), ARTN (0. 21 nM), or artefin (1.4 μM) for 24 h. The results are presented as percentage of control (CGNs transfected with empty vector) set at 100%, and the error bars indicate SEM. Student’s paired t-test was used for evaluation. +Presents p-values for comparison of the neuritogenic effect of compounds to the untreated neurons, while ∗ shows p-values for comparison to the positive control of CGNs transfected with empty vector. +p < 0.05; +++p < 0.001; ++++p < 0.0001; ∗p < 0.05; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. (E) Micrographs of neurite outgrowth of CGNs transfected with dnFGFR and incubated with media alone, P2-d, ARTN, and artefin. Scale bar: 100 μm.