Table I.

Gametocyte- and Sex-Specific Transcripts Currently Used in Molecular Detection Methods

Gene ID name	Limit of detection	Description/putative function	FG:MG ratioa	Remarks	Refs
PF3D7_1031000 Pfs25	0.01 FG/μl	Zygote and ookinete surface protein, necessary for infectivity, transmission-blocking vaccine candidate	35.58		37, 112
PF3D7_0630000 No alias	0.3 gametocytes/μl	CPW-WPC family protein	47.83	Contains nine introns	[111]
PF3D7_1351600 PfGK	0.3 FG/μl	Glycerol kinase	41.41		[43]
PF3D7_0903800 CCp4	0.1 FG/μl	LCCL domain-containing protein, sexual-stage adhesion	38.49	Contains one intron	[44]
PF3D7_0208900 Pfs230p	1.8 MG/μl	Gamete surface protein, knock out has no effect on fertilization	0.02		[40]
PF3D7_1469900 PfMGET	0.01 MG/μl	Conserved Plasmodium protein, unknown function	0.02	Contains two introns	[34]
PF3D7_1311100 Pf13	22 MG/μl	Meiosis-specific nuclear structural protein	0.005		[43]
PF3D7_1319800 Pfg17	0.1 gametocytes/μl	Conserved Plasmodium protein, unknown function	0.60	Total gametocyte marker	[113]

^aRatio of transcript expression as detected by RNAseq in sex-sorted gametocytes, expressed as FPKM (fragments per kilobase million) values in female over male gametocytes [41].