Figure 5.
Calcitonin receptor-like receptor and NMDA receptor were required for CGRP-induced LTP in the ACC. (a) Bath applied CGRP1 receptor antagonist CGRP8-37 (1 μM) completely blocked CGRP (100 nM)-induced LTP in the ACC slices (n = 6 slices/4 mice). (b) Superimposed polygonal diagrams of the activated channels in the baseline state (blue) and after the application of CGRP8-37 + CGRP (red) in six slices from four wild-type mice. (c) Bath applied NMDA receptor antagonist AP-5 (50 μM) blocked CGRP-induced LTP in the ACC slices (n = 6 slices/4 mice). (d) Superimposed polygonal diagrams of the activated channels in the baseline state (blue) and after the application of AP-5 + CGRP (red) in six slices from four wild-type mice. (e) Summarized results of the CGRP8-37 and AP-5 on CGRP-induced LTP in the ACC. (f) There were no significant enlarged areas after the application of CGRP in the presence of CGRP8-37 and AP-5 in the ACC. The mean slopes of fEPSPs were determined within last 30 min of 4 h LTP recording. All antagonists were applied 30 min before the application of CGRP and washed out together. The dashed line indicated the mean basal synaptic responses. *P < 0.05 and **P < 0.01 mean antagonist groups versus CGRP group. n.s. means no significant difference, error bars indicated SEM.