Figure 7.

MEG3 Functions as a ceRNA with miR-27a to Regulate PHLPP2 Expression

(A) The miR-27a expression level was analyzed with qRT-PCR. U6 was used as the loading control. The results are presented as relative miR-27a levels. The bars represent the mean ± SD of triplicates. (B) The miR-27a expression level was analyzed with qRT-PCR. Input miR-27a was used as the loading control. The results are presented as relative miR-27a levels. The asterisk indicates a significant difference compared with the vector transfectant (p < 0.05). The bars represent the mean ± SD of triplicates. (C) A schematic diagram of the mechanisms underlying MEG3 functions as a ceRNA that regulates PHLPP2 expression in both normal cells and bladder cancer cells. (D and E) T24T(Vector) or T24T(MEG3) transfectants were subjected to lung metastatic assay in nude mice. Representative images of the histologic lung metastases were analyzed using H&E staining (D) and the number of lung metastatic tumors were graphed (E). The asterisk indicates a significant reduction of lung metastatic tumor by ectopic MEG3 expression, compared with the vector transfectant (p < 0.05). The bars represent the mean ± SD. (F) A schematic diagram of the mechanisms underlying MEG3’s function as a ceRNA that inhibits invasion and lung metastasis of bladder cancers.