Skip to main content
. 2019 Feb 7;9(3):527–537. doi: 10.1002/2211-5463.12594

Figure 2.

Figure 2

SLC34A2 promotes the stemness of neuroblastoma cells. (A) Representative image of spheroids formed by SH‐SY5Y cells. Scale bar, 100 μm. (B,C) SLC34A2 expression was detected in SH‐SY5Y cells and spheroids. (D,E) Expression of stemness markers (ALDH1 and Nanog) was examined in SH‐SY5Y cells and spheroids. (F,G) Expression of stemness markers was determined in SH‐SY5Y cells with or without SLC34A2 overexpression. (H) The capacity of spheroid formation was evaluated in cells depicted in (F). Scale bar, 100 μm. (I,J) Expression of stemness markers was measured in SH‐SY5Y cell spheroids with or without SLC34A2 knockdown. (K) The capacity of spheroid formation was detected in spheroids described in (I). (L,M) The overexpression and knockdown efficiency of SLC34A2 was confirmed in SH‐SY5Y cells and spheroids, respectively. The difference was assayed using one‐way ANOVA with the Tukey–Kramer post hoc test. Data are presented as mean ± SD; n ≥ 3, **P < 0.01 vs control.