Figure 4.

MiR‐25 directly binds to Gsk3β 3′UTR and thus activates Wnt signaling. (A,B) Gsk3β mRNA (A) and protein (B) levels were determined in SH‐SY5Y cells with or without miR‐25 overexpression. (C,D) Expression of Wnt3a and β‐catenin was examined in SH‐SY5Y cells with or without miR‐25 overexpression. (E) Luciferase reporter assay was performed in SH‐SY5Y cells with or without miR‐25 overexpression. (F) RIP analysis was carried out in SH‐SY5Y cells with or without Luc‐Gsk3β‐3′UTR or Luc‐Gsk3β‐3′UTR‐mut overexpression. The difference was assayed using one‐way ANOVA with the Tukey–Kramer post hoc test. Data were presented as mean ± SD; n ≥ 3, **P < 0.01 vs control.