Figure 3.

The effect on invasion ability of bladder cancer cells with knockdown of circLPAR1 by siRNA. (A) The design of siRNAs against circLPAR1, in which removing joints can break the ring structure. (B) circLPAR1 and LPAR1 RNA expression following treatment with two siRNAs in 293 cells by reverse transcription-quantitative polymerase chain reaction analysis. The invasion ability of (C) T24 and (D) 5637 cells was significantly enhanced following the knockdown of circLPAR1. The invasion ability was more enhanced for (E) T24 and (F) 5637 cells by treatment with siRNA against circLPAR1, compared with the NC (magnification, ×40). NC, negative control; siRNA, small interfering RNA; circLPAR1, circular lysophosphatidic acid receptor 1.