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. 2019 Feb 1;17(3):3305–3313. doi: 10.3892/ol.2019.10007

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Annexin V-FITC/PI staining assay. Representative graphs obtained by flow cytometric analysis following double-staining with Annexin V-FITC/PI. Compared with (A) the control cells (0 µM, 7.9±1.25%), the percentage of apoptotic U-2OS cells was increased to 28.5±0.9, 35.4±1.4 and 48.5±1.6% following treatment with (B) celastrol, (C) cisplatin and (D) celastrol combined with cisplatin for 48 h, respectively. (E) Quantification of parts A-D. Data are expressed as means ± standard deviation. ***P<0.001 vs. control (0 µM). FITC, fluorescein isothiocyanate; PI, propidium iodide.