Figure 2.

Treatment with 3 µM nintedanib for 4 weeks promoted Pca cell entosis. (A) Entosis developed when Pca cells were treated with nintedanib. Engulfing cells were morphologically transformed into phagocytes with pseudopodia, a multipolar shape and multiple nuclei (magnification, ×200). (B) β-catenin expression was increased compared with the cells without nintedanib treatment. (C) Fluorescence images of 22RV1 and DU145 cells treated with nintedanib. Cells were incubated with antibody against RhoA and visualized under a fluorescence microscope (magnification, ×400). The immunofluorescence images revealed entosis-like changes. Pca, prostate cancer; C, control; T, treatment.