Figure 3.

Nintedanib induced the ROCK pathway and E-cadherin expression (3 µM for 4 weeks) by western blot analysis and the reverse transcription- quantitative polymerase chain reaction. (A) ROCK pathway-associated molecule expression following nintedanib treatment. (B) E-cadherin expression following nintedanib treatment. (C) Treatment of PC3 cells with nintedanib increased ROCK1/2 and E-cadherin expression. (D) Buparlisib treatment induced Pca cell entosis and increased ROCK1/2 and E-cadherin expression, whereas CDC42 levels decreased in Pca cells (1.5 µM, treatment for 4 weeks, ×400 magnification). *P<0.05 and **P<0.01 vs. control (Student's t-test). ROCK, Rho kinase; E-cadherin, epithelial cadherin; Pca, prostate cancer; CDC42, cell division cycle 42; C, control; T, treatment.