Figure 5.

CDC42 overexpression blocks entosis in Pca cells. (A) Overexpression of CDC42 in Pca cells at the mRNA and protein level. *P<0.05 and **P<0.01 vs. control (Student's t-test). (B) CDC42 overexpression decreased ROCK1/2 and E-cadherin expression at the mRNA and protein levels. The ROCK inhibitor Y-27632 (10 µM for 1 week) was used as a control. *P<0.05 and **P<0.01 vs. control (one-way analysis of variance followed by Fisher's least-significant difference test). (C) Effects of CDC42 overexpression on entosis in vitro following 4 weeks of nintedanib pressure (3 µM). A total of 500 randomly selected cells per condition were scored. *P<0.05 vs. control (Student's t-test). CDC42, cell division cycle 42; Pca, prostate cancer; ROCK, Rho kinase; E-cadherin, epithelial cadherin; C, control; +, CDC42+; Y, Y-27632.