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. 2019 Jan 21;17(3):3219–3226. doi: 10.3892/ol.2019.9955

Figure 4.

Figure 4.

Corroboration of Wnt7a as a direct target of miR-127. (A) Schematic diagram of the binding sites of miR-127 with the 3′-UTR of Wnt7a. (B) Relative luciferase activities in two gastric cancer (GC) cell lines after transfection with miR-127 mimic in wild-type or mutant type. (C) Analysis of Wnt7a mRNA expression in GC cells after transfection with Wnt7a siRNA by RT-qPCR. (D) Analysis of Wnt7a protein level in GC cells after transfection with Wnt7a siRNA evaluated by western blot analysis. *P<0.05; #P<0.05.