Table II.
cDNA | Genbank accession no. | Primer | Primer sequence (5′-3′) | Position | PCR product size (bp) |
---|---|---|---|---|---|
ZFP91 | NM_053023 | S | TGTCCTTGCCCATCCTCGCTA | 1,128–1,148 | 190 |
A | ACTCTTGAAGGCCCGAGCAC | 1,298–1,317 | |||
TUBA1B | NM_006082 | S | TGGAACCCACAGTCATTGATGA | 430–451 | 135 |
A | TGATCTCCTTGCCAATGGTGTA | 543–564 | |||
ALAS1 | NM_000688 | S | AGACATAACATCTACGTGCAA | 2,031–2,051 | 167 |
A | GAATGAGGCTTCAGTTCCA | 2,179–2,197 | |||
B2M | NM_004048 | S | CAGCCCAAGATAGTTAAGTG | 385–404 | 262 |
A | CCCTCCTAGAGCTACCTGT | 628–646 | |||
ACTB | NM_001101 | S | CAGCCATGTACGTTGCTATCCAG | 473–496 | 151 |
A | GAGGTCCAGACGCAGGATGGCATG | 601–623 | |||
HPRT1 | NM_000194 | S | CTCCTCTGCTCCGCCACCG | 103–121 | 218 |
A | TCGAGCAAGACGTTCAGTCC | 301–320 |
S, sense; A, antisense; ZFP91, zinc finger protein 91; TUBA1B, tubulin α1b; ALAS1, aminolevulinate, δ-synthase; B2M, β2-microglobulin; ACTB, β-actin; HPRT1, hypoxanthine phosphoribosyltransferase 1; PCR, polymerase chain reaction.