Figure 1.

Induction of MASTL expression in HepG2 and SUN387 cells. Cells were stimulated with (A) TNFα and (B) IL-6. Examination of relative MASTL mRNA expression in HepG2 cells treated with TNF-α or IL-6 (100 pg/ml) at the indicated time points by reverse transcription-quantitative polymerase chain reaction. The expression of MASTL in HepG2 and SUN387 cells were measured (C and D), and β-actin was used as an internal control. Data are representative of three independent experiments. Results are displayed as the mean ± standard error of the mean. *P<0.05, **P<0.01 vs. respective control. MASTL, microtubule associated serine/threonine kinase-like; TNF-α, tumor necrosis factor α; IL-6, interleukin 6.