Figure 3.

Effects of MASTL overexpression on the proliferation of liver cancer cell lines HepG2 and SUN387. (A) Overexpression of MASTL in HepG2 and SUN387 cells was confirmed by western blot analysis. Transfection of an empty vector was used as a control, and β-actin was used as a loading control. (B) An MTT assay was performed following overexpression of MASTL at the indicated time points. (C) Staining of WT or kinase-dead mutant MASTL expression with anti-flag in HepG2 cells by immunofluorescence (magnification, ×100). (D) Cell cycle progression of HepG2 cells in overexpressing WT or mutant MASTL was examined using fluorescence-activated cell sorting. (E) The percentages of cells in the respective cell cycle phases. Experiments were repeated three times. Results are displayed as the mean ± standard error of the mean. *P<0.05, **P<0.01 vs. respective controls. MASTL, microtubule associated serine/threonine kinase-like; WT, wild-type.