Figure EV1. Subcellular localization of m152 mutant proteins in 293T cells.

1. 293T cells were co‐transfected with expression plasmids for Cherry‐STING, V5‐tagged m152, or the respective m152 mutant (as described in Fig 3A) and either ev (unstimulated) or cGAS‐GFP (stimulated). Twenty‐four hours post‐transfection, cells were fixed for immunolabeling with an anti‐V5 antibody. Scale bar represents 10 μm.
2. 293T cells were co‐transfected with expression plasmids for Cherry‐STING, IFNβ‐Luc, pRL‐TK and either CD4, m152 or the m152‐N83Q‐N230Q‐N263Q mutant. For stimulation, samples were co‐transfected with cGAS‐GFP whereas unstimulated samples were co‐transfected with IRES‐GFP. A dual‐luciferase assay was performed. Data are combined from three independent experiments and shown as mean ± SD.