Figure 2. STING and the MCMV m152 protein co‐localize and interact under unstimulated and stimulated conditions.

• A, B
  (A) HeLa cells were co‐transfected with expression plasmids for Cherry‐STING, V5‐tagged m152, and either ev (unstimulated) or cGAS‐GFP (stimulated). (B) iMEF^gt/gt were co‐transfected with expression plasmids for V5‐tagged m152 together with either Cherry‐STING or ev in combination with cGAS‐GFP (stimulated) or ev (unstimulated). Twenty‐four hours post‐transfection, cells were fixed for immunolabeling with an anti‐V5 antibody. White boxes indicate the region shown at a higher magnification. Scale bar represents 10 μm.
• C
  Lysates of Cherry‐STING and either CD4‐V5 or m152‐V5 expressing 293T cells were subjected to immunoprecipitation (IP) with an anti‐V5 antibody. Input and IP samples were analyzed by IB with the indicated antibodies.
• D
  iMEF stably expressing ev or m152‐V5 were left unstimulated or stimulated with 10 μg/ml ISD and lysed 90 min later. m152 was immunoprecipitated with an anti‐V5 antibody, and samples were analyzed by IB with V5, STING, and phospho‐TBK1 (pTBK1)‐specific antibodies.

Data information: IB shown are representative of three (C) or two (D) independent experiments.Source data are available online for this figure.