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. 2019 Jan 29;38(5):e100983. doi: 10.15252/embj.2018100983

Figure EV2. m152 delays the translocation of STING and downstream signaling.

Figure EV2

  1. Representative still images from live cell imaging experiments with iMEFgt/gt stably expressing Cherry‐STING and either m152‐V5 or empty vector (ev) transfected with ISD for 120 min (right panel) or left unstimulated (left panel).
  2. iMEF stably expressing m152‐V5 and respective control cells (ev) were mock stimulated or stimulated with 10 μg/ml ISD in the presence or absence of Brefeldin A. 90 min post‐stimulation, cell lysates were analyzed by SDS–PAGE under non‐reducing conditions and subjected to immunoblotting with antibodies specific to STING and phospho‐TBK1.
  3. iMEF stably expressing V5‐tagged m152 or corresponding control cells (ev) were stimulated with 5 μg/ml ISD for the indicated time or left unstimulated (mock). Lysates were subjected to IB with the specified antibodies. IB shown are representative of two independent experiments.

Source data are available online for this figure.