Figure EV3. STING trafficking and downstream signaling is delayed in iMEF infected with parental MCMV compared to infection with MCMV m152stop.

1. iMEF were infected by centrifugal enhancement with parental MCMV at an MOI of 0.5 or mock infected. Cells were lysed at the indicated time points, and lysates were subjected to immunoblotting with antibodies specific to the MCMV proteins immediate‐early protein 1 (IE1) and m152. Tubulin levels were determined with a tubulin antibody.
2. Representative still images from live cell imaging experiments with iMEF^gt/gt stably expressing Cherry‐STING infected with the parental MCMV (upper panel) or MCMV m152stop (lower panel) at 120 min post‐infection.
3. iMEF were infected by centrifugal enhancement with parental MCMV or MCMV m152stop at an MOI of 0.1 or mock infected. Cells were lysed at the indicated time points, and lysates were subjected to immunoblotting with specified antibodies.
4. iMEF or iMEF^gt/gt were infected by centrifugal enhancement with parental MCMV at an MOI of 0.01. Six hpi, total RNA was extracted and m152 transcript levels were determined by qRT–PCR. Data were normalized to 10⁷ cellular β‐actin transcripts and are shown as mean ± SD.

Source data are available online for this figure.