Figure 1.

(a) Design of a general enzymatic platform for synthesis of chiral α-CF₃ organoborons. (b) On the left, overlay of Rma cyt c (PDB 3CP5, front loop region in blue), Rma TDE (PDB 6CUK, front loop region in orange), and Rma TDE with iron porphyrin carbene structure obtained from computational modeling (ref (52), front loop region in green). The large change in front loop structure highlights the impact loop mutations have on access to the active site of Rma cyt c. On the right, we propose that the active-site environment can be tuned to orient the heme-carbene intermediate such that the alkyl substituent R is solvent-exposed.