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. 2019 Jan 28;5(2):277–289. doi: 10.1021/acscentsci.8b00688

Figure 4.

Figure 4

Transfection efficiency test of F-DMA/CCL19. DMA/F-DMA containing pEGFP (4 μg) was used to transfect CT26 cells at a weight ratio of 1:50 pEGFP to DMA and F-DMA. The transfection efficiency was measured by fluorescence microscopy (A) and flow cytometry after 24 h (mean ± SEM, n = 3; * p < 0.05, **p < 0.01; F-DMA/GFP versus DMA/GFP) (B). Detection of CCL19 in cell supernatant from different groups by ELISA (mean ± SEM, n = 3; *, p < 0.05, **, p < 0.01; F-DMA/CCL19 versus DMA/CCL19; F-DMA/CCL19, DMA/CCL19 versus GS, DMA, DMA/pVax) (C). Scale bar is 200 μm. The results represent three independent experiments.