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. 2019 Mar;29(3):396–406. doi: 10.1101/gr.232330.117

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© 2019 Sanchez et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Validation of conserved essential and nonessential genes. (A) Mapped chromosomal insertion positions are plotted across Chromosome V. Haploid inserts are indicated in red; diploid inserts, in blue; and overlapping inserts, in purple. Genes indicated across the top are outlined according to predicted dispensability and filled in if confirmed. (B) Tetrad analysis of a confirmed conserved essential gene brr2Δ in S. cerevisiae and S. uvarum. Segregants containing brr2Δ alleles are inviable in both species. (C) Table indicating the insertion ratio (number of haploid inserts by the number of diploid inserts) per gene. The final column lists the predicted classification: (NE) nonessential; (E) essential; (N/A) no data.