Figure 6.

Complementation assay confirming two examples of genes that differ in essentiality but complement the viability phenotype in both genetic backgrounds. Letters a through d represent four spores, whereas numbers 1 through 3 indicate three tetrads. Tetrads were dissected on YPD and were replica plated to G418 to indicate which spore contains the deletion and to C-URA to indicate the presence of the plasmid expressing the indicated gene. (A) S. cerevisiae lcd1Δ::KanMX strain containing a plasmid with the S. uvarum allele of LCD1. (B) S. uvarum aft1Δ::KanMX strain containing a plasmid with the S. cerevisiae allele of AFT1.