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. 2019 Mar;29(3):439–448. doi: 10.1101/gr.241414.118

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© 2019 Sherill-Rofe et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Screen for germline radiation sensitivity in C. elegans. L1 worms were subjected to RNAi of the indicated genes by feeding. Adults were exposed to 50 Gy. After 2 h of recovery, 15 worms were replated with one worm per plate, and 15 nonirradiated worms were plated as controls. After 36 h, the number of eggs and larvae were recorded. The number of progeny is presented relative to worms fed empty-vector containing bacteria. rad-51, a known HRR gene in C. elegans, served as a positive control.