Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar;29(3):439–448. doi: 10.1101/gr.241414.118

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Sherill-Rofe et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 5. — (Top) The DR-GFP reporter assay. SceGFP is a modified version of the GFP gene, which contains an I-Sce site and an in-frame premature stop codon that can be removed by HR-mediated repair of the I-SceI–induced DSB using the internal GFP fragment (iGFP) placed downstream from the SceGFP cassette. (Middle) The experimental setup used to monitor HRR in the HeLa DR-GFP cell line. (Bottom) The percentage of GFP+ cells for each experimental condition (siRNA +/− I-SceI) was measured by flow cytometry (FACS) following the experimental procedure detailed in the top right panel and normalized to the siCtrl + I-SceI conditions, in two human cell lines: HeLa and U2OS. (***) P < 0.0001, (**) P < 0.001, (*) P < 0.01, (ns) nonsignificant.