Fig. 2.

Number of HLA-I and HLA-II binding alleles by each predicted epitopes. Predicted epitopes with similarity less than 60% with structural and nonstructural proteins from other flaviviruses and theoretical number of binding HLA-I or HLA-II for each epitope are shown. Dotted line represents cutoff value (four or more binding HLA-I or HLA-II alleles). Only epitopes above cutoff were submitted to antigenicity calculation through VaxiJen server version 2.0 (http://www.ddg-pharmfac.net/vaxijen/VaxiJen/VaxiJen.html). HLA, human leukocyte antigen.