Figure 4.
miR-34a/c-5p and miR-449a/b were predicted to be candidate miRNAs. (A) Venn diagram of overlapped binding miRNAs. mircode and microRNA.org were used to predict MALAT1 and c-Met and SOX4 binding miRNAs, respectively. (B) Relative expression of miR-34a was detected using qRT-PCR in 76 osteosarcoma tissues and paired adjacent non-tumor tissues. (C) Relative expression of miR-34c-5p was detected using qRT-PCR in 76 osteosarcoma tissues and paired adjacent non-tumor tissues. (D) Relative expression of miR-449a was detected using qRT-PCR in 76 osteosarcoma tissues and paired adjacent non-tumor tissues. (E) Relative expression of miR-449b was detected using qRT-PCR in 76 osteosarcoma tissues and paired adjacent non-tumor tissues. Data are presented as mean ± standard deviation for 3 independent experiments. ** P<0.01 compared with non-tumor tissues.