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. 2019 Feb 22;25:1410–1422. doi: 10.12659/MSM.912703

Figure 5.

Figure 5

MALAT1 functioned as a ceRNA of c-Met and SOX4 by directly binding to miR-34a/c-5p and miR-449a/b. (A) Putative binding sites of miR-34a, miR-34c-5p, miR-449a, and miR-449b in c-Met 3′UTR, SOX4 3′UTR, and MALAT1. (B) Dual-luciferase reporter assay in MG-63 cells, co-transfected of wild type (WT) or mutant (Mut) of MALAT1, c-Met 3′ UTR,or SOX4 3′UTR with miR-34a, miR-34c-5p, miR-449a, and miR-449b mimics or miRNA negative control. ** P<0.01 compared with miRNA negative control. ## P<0.01 compared with Mut vectors. (C) c-Met mRNA and protein expression level in MG-63 cells co-transfected with MALAT1 siRNA and miR-34a, miR-34c-5p, miR-449a, or miR-449b inhibitor. ** P<0.01 compared with siRNA control. (D) SOX4 mRNA and protein expression level in MG-63 cells co-transfected with MALAT1 siRNA and miR-34a, miR-34c-5p, miR-449a, or miR-449b inhibitor. ** P<0.01 compared with siRNA control. All data are presented as mean ± standard deviation for 3 independent experiments.