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. 2019 Feb 28;15:68. doi: 10.1186/s12917-019-1813-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of TNFR-Igs on cell death by TNF-α L929 cells were cultured with recombinant bovine TNF-α, actinomycin-D, and TNFR1-Ig, TNFR2-Ig, or Cont-Ig under several concentrations. Cell viabilities were assessed using the RealTime-Glo™ MT Cell Viability Assay according to the manufacturer’s instructions. Both TNFR-Ig, but particularly TNFR2-Ig, significantly reduced cell death induced by TNF-α. The assays were conducted in triplicate. * p < 0.05