Fig. 8.

Schematic representation of the signalling cascade involved in the formation of GroPIns4P in NIH3T3 cells. In NIH3T3 cells, activation of the EGF receptor (EGFR) can lead to activation of cPLA₂α through the involvement of MEK kinase. cPLA₂α activation results in hydrolysis of PtdIns4P and release of GroPIns4P, which binds to Shp1 and promotes its association with Src. Once activated Src triggers a signalling cascade culminating with the formation of Tiam/Rac complex at the plasma membrane and consequent formation membrane ruffles with stimulation of cell motility. See text for details