Table 5.
Differentially methylated regions in blood DNA in relation to NO2 exposure (adjusted p value < 0.05 both in DMRcate and in comb-p)
| Chra | Gene (distance to geneb) | DMRcate | comb-p | Minimum Pg | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Start (bpc) | End (bp) | FDRd | #CpGse | Start (bp) | End (bp) | Sidak Pf | #CpGs | |||
| 1 | RUNX3 | 25291041 | 25291905 | 0.005 | 7 (4) | 25291584 | 0.044 | 6 (3) | 0.001 | |
| 1 | RPS6KA1 | 26855423 | 26855926 | 0.006 | 4 (3) | 26855765 | 0.009 | 3 (3) | 9.1E-04 | |
| 1 | TFAP2E | 36038468 | 36039173 | 2.1E-04 | 8 (7) | 36038701 | 3.3E-04 | 6 (6) | 0.002 | |
| 1 | ARTN | 44398868 | 44399894 | 5.6E-04 | 10 (6) | 44399363 | 0.012 | 6 (4) | 9.8E-04 | |
| 1 | S100A12 h | 153347819 | 153348305 | 2.8E-04 | 2 (2) | 0.005 | 2.7E-06 | |||
| 1 | S100A14 | 153589528 | 153590243 | 0.013 | 4 (2) | 153589781 | 0.047 | 3 (2) | 0.001 | |
| 1 | S100A13 | 153599479 | 153600156 | 0.001 | 7 (6) | 6.6E-04 | 0.003 | |||
| 1 | ATP8B2 | 154300117 | 154300241 | 6.9E-04 | 2 (2) | 0.007 | 3.4E-05 | |||
| 1 | LAX1 | 203733971 | 203734559 | 0.004 | 6 (4) | 0.002 | 0.002 | |||
| 1 | C1orf35 | 228291118 | 228291705 | 0.023 | 6 (5) | 0.017 | 0.009 | |||
| 2 | ALS2CR11 | 202483704 | 202484583 | 0.007 | 10 (5) | 202484020 | 0.008 | 7 (5) | 0.006 | |
| 3 | AMT | 49459143 | 49460521 | 1.3E-06 | 10 (7) | 49459855 | 8.3E-05 | 9 (7) | 1.6E-04 | |
| 3 | PPM1L | 160475035 | 160475336 | 0.002 | 5 (5) | 0.003 | 0.002 | |||
| 3 | B3GALNT1 | 160822268 | 160822911 | 0.001 | 8 (5) | 160822711 | 0.031 | 5 (4) | 0.003 | |
| 5 | MGAT4B | 179230709 | 179231109 | 0.002 | 3 (2) | 0.006 | 2.5E-04 | |||
| 5 | OR2V1 (− 39,287) | 180511822 | 180512070 | 0.012 | 2 (2) | 0.020 | 7.8E-04 | |||
| 6 | DUSP22 | 291687 | 292823 | 7.6E-04 | 9 (8) | 291882 | 2.2E-04 | 8 (7) | 0.005 | |
| 6 | TRIM39 | 30297174 | 30297627 | 1.5E-06 | 11 (9) | 2.9E-05 | 6.7E-04 | |||
| 6 | LTA h | 31539539 | 31540750 | 1.9E-15 | 19 (11) | 31540461 | 4.5E-07 | 18 (11) | 3.5E-06 | |
| 6 | HLA-DMB | 32904074 | 32905190 | 1.2E-05 | 9 (5) | 32904621 | 0.001 | 5 (3) | 8.7E-06 | |
| 6 | HLA-DPB2 | 33083989 | 33085470 | 2.5E-06 | 22 (12) | 33084420 | 2.3E-04 | 21 (11) | 0.006 | |
| 6 | TRAF3IP2 | 111887243 | 111887834 | 0.002 | 2 (2) | 0.026 | 3.2E-04 | |||
| 6 | MLLT4; C6orf124 | 168227843 | 168228706 | 0.001 | 3 (3) | 168228374 | 0.004 | 2 (2) | 6.4E-05 | |
| 7 | UNCX (− 5426) | 1266180 | 1267228 | 8.2E-04 | 4 (4) | 1266616 | 0.001 | 3 (3) | 2.0E-04 | |
| 7 | EVX1 (− 2589) | 27279101 | 27279575 | 0.009 | 3 (2) | 0.044 | 1.8E-04 | |||
| 7 | STEAP2 | 89840396 | 89841435 | 1.9E-05 | 13 (5) | 89841214 | 0.004 | 12 (5) | 2.1E-04 | |
| 8 | CSGALNACT1 | 19459672 | 19460243 | 2.3E-05 | 7 (5) | 6.7E-05 | 1.4E-04 | |||
| 8 | KIAA0146; CEBPD (−19) | 48648112 | 48649767 | 7.6E-08 | 7 (7) | 48648813 | 3.9E-09 | 6 (6) | 7.4E-05 | |
| 8 | HEY1 | 80678770 | 80679314 | 0.002 | 4 (3) | 80678925 | 0.026 | 2 (2) | 4.4E-04 | |
| 8 | NDRG1 | 134307105 | 134307728 | 2.3E-05 | 3 (3) | 134307597 | 7.4E-04 | 2 (2) | 3.0E-05 | |
| 10 | HK1 | 71087924 | 71088038 | 0.009 | 2 (2) | 0.038 | 2.0E-04 | |||
| 10 | LRRC20 | 72141375 | 72141924 | 7.0E-06 | 5 (3) | 72141625 | 0.007 | 4 (3) | 1.2E-05 | |
| 10 | HTRA1 | 124254773 | 124254860 | 0.003 | 2 (2) | 0.010 | 1.1E-04 | |||
| 11 | IFITM3 (10863) | 330536 | 331179 | 5.1E-04 | 5 (3) | 0.001 | 1.2E-05 | |||
| 11 | LMO2 | 33913187 | 33914088 | 9.3E-04 | 5 (4) | 3.2E-04 | 7.2E-04 | |||
| 11 | ME3 (− 9563) | 86142104 | 86142587 | 5.1E-04 | 4 (3) | 0.012 | 1.0E-04 | |||
| 13 | PDX1 (− 1903) | 28491326 | 28492265 | 0.006 | 8 (3) | 28491409 | 28491975 | 0.035 | 6 (3) | 0.001 |
| 13 | PCDH20 | 61989203 | 61990025 | 6.0E-05 | 12 (8) | 61989701 | 5.3E-04 | 8 (7) | 6.7E-04 | |
| 13 | DAOA (−319,060) | 105791890 | 105792346 | 0.023 | 3 (3) | 0.024 | 0.003 | |||
| 14 | DAD1 (−58,286) | 22974144 | 22975521 | 0.007 | 6 (5) | 22974951 | 0.029 | 5 (4) | 1.2E-04 | |
| 14 | CTSG | 25045625 | 25046121 | 0.013 | 3 (3) | 0.008 | 0.002 | |||
| 14 | PLD4 h | 105390602 | 105391263 | 0.002 | 3 (2) | 105391018 | 0.007 | 2 (2) | 3.0E-06 | |
| 15 | GATM; LOC145663h | 45670068 | 45671708 | 8.3E-08 | 17 (7) | 45670478 | 45671347 | 1.2E-04 | 14 (7) | 6.4E-07 |
| 15 | TNFAIP8L3 | 51387571 | 51387921 | 0.002 | 5 (5) | 0.004 | 9.7E-04 | |||
| 15 | FLJ42289 | 100890441 | 100891257 | 8.3E-07 | 9 (4) | 100890907 | 1.0E-05 | 5 (4) | 3.3E-05 | |
| 16 | TMEM8A; LOC100134368 | 432973 | 434356 | 1.3E-05 | 7 (4) | 433439 | 433825 | 1.1E-04 | 5 (4) | 1.2E-04 |
| 16 | CLDN9 | 3062056 | 3062975 | 0.001 | 8 (6) | 3062349 | 8.0E-04 | 7 (6) | 0.005 | |
| 17 | ALOX12 | 6898738 | 6900356 | 6.9E-10 | 16 (12) | 6899888 | 1.9E-08 | 15 (12) | 0.001 | |
| 17 | WNK4 | 40932199 | 40932983 | 0.006 | 11 (6) | 40932746 | 0.005 | 9 (6) | 0.011 | |
| 17 | IGF2BP1 | 47091521 | 47092272 | 0.006 | 6 (5) | 47091978 | 0.042 | 5 (4) | 0.003 | |
| 17 | CCDC57 | 80084554 | 80085082 | 0.003 | 4 (4) | 0.001 | 0.002 | |||
| 19 | ELANE | 855536 | 856107 | 4.5E-04 | 4 (4) | 6.1E-05 | 2.8E-04 | |||
| 19 | FBXO17 | 39465821 | 39467258 | 2.0E-04 | 9 (6) | 39466757 | 6.1E-05 | 8 (6) | 0.003 | |
| 20 | C20orf123 | 45179157 | 45179413 | 0.002 | 6 (5) | 0.005 | 0.002 | |||
| 21 | RUNX1 | 36259067 | 36259797 | 0.005 | 5 (4) | 36259460 | 0.008 | 4 (4) | 0.003 | |
| 22 | PARVG | 44568337 | 44568812 | 0.024 | 9 (5) | 0.043 | 0.006 | |||
| 22 | PRR5 | 45125218 | 45126040 | 0.002 | 5 (4) | 45125666 | 0.005 | 4 (3) | 0.002 | |
Blanked cells in “Start,” “End,” and “#CpGs” for comb-p represent the same information compared to results in DMRcate
aChromosome
bMinimum distance to transcription start site of the mapped gene (base pair)
cPhysical position (base pair, National Center for Biotechnology Information human reference genome assembly Build 37.3)
dBenjamini-Hochberg false discovery rate
eNumber of probes in the region (number of probes having uncorrected p value < 0.05)
fP of Sidak multiple-testing correction
gMinimum p value among uncorrected p-values of CpGs in the region. When either start or end positions were different between DMRs from the two DMR approaches, we used results from DMRcate
hRegion including significant (FDR < 0.05) differentially methylated probes from our epigenome-wide association study